The ImageStream X (ISX) combines the quantitative power of Flow Cytometry with high content image analysis. It quantifies both the intensity and the location of fluorescent probes and can analyse highly heterogeneous samples and rare cell subpopulations at a rate of above 1000 cells per second. The ISX provides new insights into the co-localisation of proteins, cell-cell interactions, internalisation, targeted immunotherapy, parasitaology or cell cycle/cell death related questions.
A unique six-channel CCD camera and a novel velocity detection system work in concert to collect 1,000 times more light than conventional technology. The technique is called Time Delay Integration. The result is high resolution imagery with fluorescence sensitivity superior to flow cytometry.
The ImageStream system combines a bright field lamp, and 5 excitation lasers (488 nm, 405 nm, 560 nm, 592 nm and 647 nm). Cellular imagery is split into six component colours through a unique spectral decomposition element. The result is a bright field image, a dark field image, and multiple fluorescence images of every cell. For high throughput analysis, the ISX is equipped with a 96 well plate autosampler. The ImageStream uses IDEAS software.
Channels and Filters:
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Channel 1: 430 – 505 nm
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Channel 2: 505 – 560 nm
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Channel 3: 560 – 595 nm
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Channel 4: 595 – 660 nm
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Channel 5: 660 – 745 nm
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Channel 6: 755 – 800 nm
Laser (nm) and example Dyes
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405: DAPI, Pacific Blue, Hoechst, Alexa 405
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488: FITC, GFP, YFP, PE, ECD, PE-Cy5
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560: Alexa Fluor 546, Cy3, PI, 7AAD
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592: Texas Red, Alexa Fluor 594
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658: Cy5, Alexa Fluor 647, APC, APC-Cy-7
Book time on the ImageStream X